Separation of uroporphyrin esters I and III by paper chromatography.
نویسندگان
چکیده
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منابع مشابه
Chromatographic separation of uroporphyrins I and III and the nature of Waldenström porphyrin.
Patients with porphyria usually discharge large amounts of uroporphyrins, mainly as a mixture of the isomers I and III, as well as other porphyrins. Knowledge concerning their quantitative distribution in the disease has long been sought. The chromatographic separation of methyl esters of uroporphyrin I and “Waldenstrom porphyrin” (1, 2), then believed to be uroporphyrin III, was described by F...
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5. Specimens from Prof. C. J. Watson's laboratory have been examined. The uroporphyrin methyl ester I had m.p. 292-293° and behaved as uroporphyrin I containing a trace of uroporphyrin III on paper chromatography. The 2080 ester behaved chromatographically as a hexacarboxylic porphyrin. We wish to thank Dr Falk and Miss Benson for performing the chromatographic examinations by their method, and...
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Hepta-, hexa-, and pentacarboxylic porphyrins have been detected in porphyric as well as normal urine samples. From the former they have been isolated and converted into pure methyl esters (1). For a better understanding of the chemistry of these porphyrins, pure uroporphyrin I and uroporphyrin III were separately subjected to decarboxylation under controlled conditions. The decarboxylation pro...
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Hydrochloric acid extraction of feces in the presence of ether yields an extract suitable for spectrophotometric estimation of total porphyrin and for further separation by "high-performance" liquid chromatography (HPLC) or thin-layer chromatography. A total porphyrin reference interval of less than 200 nmol/g dry weight of feces was established from data on 106 normal subjects on an unrestrict...
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An improved, specific and sensitive method for the determination of the coproporphyrin isomers I and III is described. In this method, the hydrolysis of the coproporphyrin methyl esters takes place in the silica gel layer of acid-resistant pre-coated thin layer plates. After chromatography in the solvent system 2,6-dimethylpyridine/water, (volume ratio 20 ml + 6 ml), in an ammonia atmosphere, t...
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عنوان ژورنال:
- The Biochemical journal
دوره 53 4 شماره
صفحات -
تاریخ انتشار 1953